The connection of alpha- and beta-domains in mammalian metallothionein-2 differentiates Zn(II) binding affinities, affects folding, and determines zinc buffering properties.
Singh AK, Pomorski A, Wu S, Peris-Diaz MD, Czepczynska-Krezel H, Krezel A
Metallomics. 2023 Jun 1;15(6):mfad029. doi: 10.1093/mtomcs/mfad029.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 105694 | MT2a_K30A | Bacterial expression plasmids for production of recombinant metallothionein 2a K30A mutant |
| 105695 | MT2a_K31A | Bacterial expression plasmids for production of recombinant metallothionein 2a K31A mutant |
| 105696 | MT2a_K30A K31A | Bacterial expression plasmids for production of recombinant metallothionein 2a K30/31A mutant |
| 105697 | MT2a_C21A | Bacterial expression plasmids for production of recombinant metallothionein 2a C21A mutant |
| 105698 | MT2a_K20A | Bacterial expression plasmids for production of recombinant metallothionein 2a K20A mutant |
| 105699 | MT2a_S6A | Bacterial expression plasmids for production of recombinant metallothionein 2a S6A mutant |
| 200300 | MT2A_beta-domain | Bacterial expression plasmids for production of recombinant beta-domain of metallothionein 2a |
| 200301 | MT2A_beta-Villin | Bacterial expression plasmids for production of recombinant fusion protein having beta-domain of metallothionein 2a and chicken Villin headpiece domain HP-35 |
| 200302 | MT2A_alpha-domain | Bacterial expression plasmids for production of recombinant alpha-domain of metallothionein 2a |