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LINE-1 vectors mediate recombinant antibody gene transfer by retrotransposition in Chinese hamster ovary cells.

Zheng F, Kawabe Y, Murakami M, Takahashi M, Nishihata K, Yoshida S, Ito A, Kamihira M
Biotechnol J. 2021 Jul;16(7):e2000620. doi: 10.1002/biot.202000620. Epub 2021 May 20. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
206020pLINE1-scFvFcAn intact type LINE-1 vector plasmid possessing the scFv-Fc expression unit
206021pLINE1ΔORF2-scFvFcThe ORF2 sequence was removed from pLINE1-scFvFc
206022pLINE1ΔORF1-2-scFvFcThe ORF1 and ORF2 sequences were removed from pLINE1-scFvFc
206023pLINE1mORF1-ΔORF2-scFvFcThe base next to the ORF1 start codon (ATG) was deleted in pLINE1ΔORF2-scFvFc
206024pORF1-2An expression vector for mouse LINE1 ORF1-2
206025pORF1An expression vector for mouse LINE1 ORF1
206026pORF2An expression vector for mouse LINE1 ORF2

Antibodies from Article