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Efficient Cloning of Inserts for Phage Display by Golden Gate Assembly.

Grahn AK, Allen GL, Kay BK
Methods Mol Biol. 2023;2702:191-203. doi: 10.1007/978-1-0716-3381-6_9. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
210203mTB3-AviD tagType 3 phage-display vector that contains kanamycin resistance and displays the AviD-tag at the N-terminus of mature protein III.
210204mTB3-FLAG tagType 3 phage-display vector that contains kanamycin resistance and displays the FLAG-tag at the N-terminus of mature protein III.
210205mTB3-Myc tagType 3 phage-display vector that contains kanamycin resistance and displays the Myc-tag at the N-terminus of mature protein III.
210208mTB3-BC2 nanobodyType 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.

Antibodies from Article