Uncovering the functional diversity of rare CRISPR-Cas systems with deep terascale clustering.
Altae-Tran H, Kannan S, Suberski AJ, Mears KS, Demircioglu FE, Moeller L, Kocalar S, Oshiro R, Makarova KS, Macrae RK, Koonin EV, Zhang F
Science. 2023 Nov 24;382(6673):eadi1910. doi: 10.1126/science.adi1910. Epub 2023 Nov 23.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 205962 | pHS1068 | NZ_CP025815 DinG HNH proteins (E. coli codon optimized) and CRISPR array in pACYCDuet-1 with Lac promoters |
| 205963 | pHS1543 | NZ_CP025815 DinG HNH Cascade proteins operon (E. coli codon optimized) with His14-tagged Csf3 in pET45b(+) |
| 205964 | pHS1182 | NZ_CP025815 DinG HNH crRNA expression in pCOLADuet-1 |
| 205965 | pHS1184 | NZ_CP025815 His14-TwinStrep-bdSUMO tagged DinG HNH protein (E. coli codon optimized) in pET45b(+) |
| 205966 | pHS1801 | JAGBLH010000220 Cas8-HNH full system (E. coli codon optimized) in pACYCDuet-1 with Lac promoters |
| 205967 | pHS1928 | JAGBLH010000220 Cas8-HNH operon (E. coli codon optimized) with His-tagged Cas8 in pET45b(+) |
| 205968 | pHS1929 | JAGBLH010000220 Cas8-HNH crRNA expression in pCOLADuet-1 |
| 205969 | pHS1232 | MWEE01000013 Cas5-HNH operon (E. coli codon optimized) + CRISPR array in pACYCDuet-1 Lac promoters |
| 205970 | pHS1477 | MWEE01000013 Cas5-HNH operon (E. coli codon optimized) with His14-tagged Cse2 in pET45b(+) |
| 205971 | pHS1398 | MWEE01000013 Cas5-HNH crRNA expression in pCOLADuet-1 |
| 205972 | pHS1859 | FLAG-SV40 NLS-Csy3/Cas7 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+) |
| 205973 | pHS1860 | FLAG-SV40 NLS-Cas6/Csy4 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+) |
| 205974 | pHS1861 | FLAG-SV40 NLS-Cas8-HNH from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+) |
| 205975 | pHS1862 | FLAG-SV40 NLS-Cas5 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+) |
| 205976 | pHS1871 | U6-JAGBLH010000220 Cas8-HNH DR-BpiI-DR |
| 205977 | pHS1514 | FLAG-SV40 NLS-Cas8/Cse1 from MWEE01000013 Cas5-HNH in pcDNA3.1(+) |
| 205978 | pHS1515 | FLAG-SV40 NLS-Cas11/Cse2 from MWEE01000013 Cas5-HNH in pcDNA3.1(+) |
| 205979 | pHS1516 | FLAG-SV40 NLS-Cas7 from MWEE01000013 Cas5-HNH in pcDNA3.1(+) |
| 205980 | pHS1517 | FLAG-SV40 NLS-Cas5-HNH from MWEE01000013 Cas5-HNH in pcDNA3.1(+) |
| 205981 | pHS1518 | FLAG-SV40 NLS-Cas6(Cse3/CasE) from MWEE01000013 Cas5-HNH in pcDNA3.1(+) |
| 205982 | pHS1528 | U6-MWEE01000013 Cas5-HNH DR-BpiI-DR |
| 205983 | pHS1950 | Ga0116245_10002768 T7/LacO-Cas6 + TwinStrep-Cas14 + Cas7 + His14-Cas5::pJ23119-DR-Fn spacer-DR |
| 205984 | pCX0393 | Ga0116245_10002768 T7/LacO-Cas6 + Cas14-TwinStrep + Cas7 + His14-Cas5::pJ23119-truncated DR-truncated Fn spacer |