Effective genome editing with an enhanced ISDra2 TnpB system and deep learning-predicted omegaRNAs.
Marquart KF, Mathis N, Mollaysa A, Muller S, Kissling L, Rothgangl T, Schmidheini L, Kulcsar PI, Allam A, Kaufmann MM, Matsushita M, Haenggi T, Cathomen T, Kopf M, Krauthammer M, Schwank G
Nat Methods. 2024 Sep 23. doi: 10.1038/s41592-024-02418-z.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 205631 | pKM-T4-CMV-TnpB-optimized-P2A-GFP-Puro | Expression of TnpBmax in mammalian cells |
| 205633 | pKM-U6-reRNA-acceptor-2xBbsI | Cloning and U6-drive expression of ISDra2 TnpB reRNAs |
| 205634 | pKM-U6-reRNA-mmPCSK9 | reRNA guide targeting Mus Musculus PCSK9 |
| 205635 | pKM-U6-reRNA-mmDNMT1 | reRNA guide targeting Mus Musculus DNMT1 |
| 205636 | pKM-U6-reRNA-mmSCN9A | reRNA guide targeting Mus Musculus SCN9A |
| 205637 | pKM-U6-reRNA-hsEMX1 | reRNA guide targeting Homo sapiens EMX1 |
| 205638 | pKM-U6-reRNA-hsHEKsite_1_3 | reRNA guide targeting HEKsite_1_3 |
| 205639 | pKM-U6-reRNA-hsRUNX | reRNA guide targeting Homo sapiens RUNX |