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Addgene

Engineering RNA export for measurement and manipulation of living cells.

Horns F, Martinez JA, Fan C, Haque M, Linton JM, Tobin V, Santat L, Maggiolo AO, Bjorkman PJ, Lois C, Elowitz MB
Cell. 2023 Jul 10:S0092-8674(23)00689-X. doi: 10.1016/j.cell.2023.06.013. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
205520pFH2.1_mCherryExpress mCherry (without export tag)
205521pFH2.2_mCherry-MS2x12Express mCherry-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)
205522pFH2.4_mCherry-MMLV_PsiExpress mCherry-Psi (export tag consisting of MMLV Psi)
205523pFH2.7_Gag-dZF2-MCP-P2A-eGFPExpress HIV Gag-delta-ZF2-MCP-P2A-eGFP
205524pFH2.9_MMLV_Gag-P2A-eGFPExpress MMLV Gag-P2A-eGFP
205525pFH2.10_Gag-MCP-P2A-eGFPExpress HIV Gag-MCP-P2A-eGFP
205526pFH2.11_Gag-MCP_ZF2-P2A-eGFPExpress HIV Gag-MCP_ZF2-P2A-eGFP
205527pFH2.12_GagZip-MCP-P2A-eGFPExpress HIV GagZip-MCP-P2A-eGFP
205528pFH2.13_GagZip-MCP_Zip-P2A-eGFPExpress HIV GagZip-MCP_Zip-P2A-eGFP
205529pFH2.14_GagZip-dp1dp6-MCP-P2A-eGFPExpress HIV GagZip-dp1dp6-MCP-P2A-eGFP
205531pFH2.16_MiniGagZip-MCP_Zip-P2A-eGFPExpress HIV MiniGagZip-MCP_Zip-P2A-eGFP
205532pFH2.17_GagZip-MCP-dpol-P2A-eGFPExpress HIV GagZip-MCP-dpol-P2A-eGFP
205533pFH2.18_TagBFPExpress TagBFP
205534pFH2.19_mCherry-MS2x2Express mCherry-MS2x2 (export tag consisting of 2 tandem repeats of MS2 stem-loop aptamer)
205535pFH2.20_mCherry-MS2x4Express mCherry-MS2x4 (export tag consisting of 4 tandem repeats of MS2 stem-loop aptamer)
205536pFH2.21_mCherry-MS2x6Express mCherry-MS2x6 (export tag consisting of 6 tandem repeats of MS2 stem-loop aptamer)
205537pFH2.22_mCherry-MS2x8Express mCherry-MS2x8 (export tag consisting of 8 tandem repeats of MS2 stem-loop aptamer)
205538pFH2.30_Gag-MCP-dpol-T2A-eGFPExpress HIV Gag-MCP-dpol-T2A-eGFP
205539pFH2.32_LV-mCherry-MS2x8-BCSExpress mCherry-MS2x8 with barcode
205540pFH2.94_PB-TRE3GS-Gag-MCP-T2A-eGFP-PGK-Tet3G-T2A-BlastExpress HIV Gag-MCP-T2A-eGFP in doxycycline-inducible manner, and constitutively express Tet3G transactivator and blasticidin resistance marker
205541pFH2.96_PB-EPN1-T2A-eGFP-PGK-BlastExpress EPN1-T2A-eGFP and blasticidin resistance marker
205542pFH2.97_PB-EPN1-MCP-T2A-eGFP-PGK-BlastExpress EPN1-MCP-T2A-eGFP and blasticidin resistance marker
205543pFH2.98_PB-EPN1-MCP_Myc-T2A-eGFP-PGK-BlastExpress EPN1-MCP-Myc-T2A-eGFP and blasticidin resistance marker
205544pFH2.99_PB-EPN1-MCP_I-T2A-eGFP-PGK-BlastExpress EPN1-MCP-I-T2A-eGFP and blasticidin resistance marker
205545pFH2.100_PB-EPN11-T2A-eGFP-PGK-BlastExpress EPN11-T2A-eGFP and blasticidin resistance marker
205546pFH2.101_PB-EPN11-MCP-T2A-eGFP-PGK-BlastExpress EPN11-MCP-T2A-eGFP and blasticidin resistance marker
205547pFH2.102_PB-EPN11-MCP_I-T2A-eGFP-PGK-BlastExpress EPN11-MCP-I-T2A-eGFP and blasticidin resistance marker
205548pFH2.103_PB-EPN11-MCP_p6-T2A-eGFP-PGK-BlastExpress EPN11-MCP-p6-T2A-eGFP and blasticidin resistance marker
205549pFH2.104_PB-EPN24-T2A-eGFP-PGK-BlastExpress EPN24-T2A-eGFP and blasticidin resistance marker
205550pFH2.105_PB-EPN24-MCP-T2A-eGFP-PGK-BlastExpress EPN24-MCP-T2A-eGFP and blasticidin resistance marker
205551pFH2.106_PB-EPN24-MCP_I-T2A-eGFP-PGK-BlastExpress EPN24-MCP-I-T2A-eGFP and blasticidin resistance marker
205552pFH2.107_PB-EPN24-MCP_p6-T2A-eGFP-PGK-BlastExpress EPN24-MCP-p6-T2A-eGFP and blasticidin resistance marker
205553pFH2.155_LV-Puro-T2A-mTagBFP2Express puromycin resistance marker and mTagBFP2
205554pFH2.156_LV-ZeoExpress zeocin resistance marker
205555pFH2.203_PB-EPN24-MCP-IRES-eGFP-PGK-BlastExpress EPN24-MCP-IRES-eGFP and blasticidin resistance marker
205556pFH2.214_mCherry-PP7x12Express mCherry-PP7x12 (export tag consisting of 12 tandem repeats of PP7 stem-loop aptamer)
205557pFH2.217_PB-EPN24-PCP-IRES-eGFP-PGK-BlastExpress EPN24-PCP-IRES-eGFP and blasticidin resistance marker
205558pFH3.2_mTagBFP2-MS2x12Express mTagBFP2-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)
205559pFH3.3_Cre-MS2x12Express Cre-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)
205560pFH3.29_CreExpress Cre (without export tag)
205561pJAM1.16_PB-Cx43_S368A-PGK-BlastExpress human Cx43 with S368A mutation
205562pJAM1.17_PB-STEAP3-PGK-BlastExpress human STEAP3
205563pJAM1.18_PB-SDC4-PGK-BlastExpress human SDC4
205564pJAM1.19_PB-CIT-PGK-BlastExpress human CIT
205565pJAM1.20_PB-NEDD4L_deltaC2-PGK-BlastExpress human NEDD4L (delta C2 isoform)
205566pJAM1.21_PB-UGCG-PGK-BlastExpress human UGCG

Antibodies from Article