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Engineering human pluripotent stem cell lines to evade xenogeneic transplantation barriers.

Pizzato HA, Alonso-Guallart P, Woods J, Connelly JP, Fehniger TA, Atkinson JP, Pruett-Miller SM, Monsma FJ Jr, Bhattacharya D
Stem Cell Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 10.1016/j.stemcr.2023.12.003. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
205443AAVS1-A2UCOE-EF1a-hCD46-P2A-HLA-G SCT-T2A-hCD47-E2A-hCD59-F2A-neo-AAVS1human AAVS1-targeting plasmid for expression of iCasp9, HLA-E SCT, human and CD55
205444AAVS1-A2UCOE-EF1a-iCasp9-P2A-HLA-E SCT-T2A-hCD55-E2A-neo-AAVS1human AAVS1-targeting plasmid for expression of human CD46, HLA-G SCT, CD47, and CD59
205445AAVS1-A2UCOE-EF1a-iCasp9-P2A-Crry-T2A-mCD59-E2A-Qa1 SCT-F2A-neo-AAVS1human AAVS1-targeting plasmid for expression of iCasp9, Crry, mouse CD59, and Qa1 SCT
205446AAVS1-A2UCOE-EF1a-HSV TK-P2A-H2-Kb SCT-T2A-mCD47-E2A-mCD55-F2A-neo-AAVS1human AAVS1-targeting plasmid for expression of HSV TK, H2-Kb SCT, mouse CD47, and mouse CD55
205447AAVS1-A2UCOE-EF1a-mCR1-F2A-neo-AAVS1human AAVS1-targeting plasmid for expression of mouse CR1
205448TRE-UBC-mCD24-T2A-GFPlentiviral plasmid for expression of mouse CD24
205449TRE-UBC-mCD47-T2A-GFPlentiviral plasmid for expression of mouse CD47
205450TRE-UBC-mCD55-T2A-GFPlentiviral plasmid for expression of mouse CD55
205451TRE-UBC-mCD59-T2A-GFPlentiviral plasmid for expression of mouse CD59
205452TRE-UBC-Crry-T2A-GFPlentiviral plasmid for expression of mouse Crry
205453TRE-UBC-Qa1 SCT-T2A-GFPlentiviral plasmid for expression of mouse Qa1 SCT
205454TRE-UBC-H2-Kb SCT-T2A-GFPlentiviral plasmid for expression of mouse H2-Kb SCT
205455TRE-UBC-mPD-L1-T2A-GFPlentiviral plasmid for expression of mouse PD-L1
205456TRE-UBC-hCD24-T2A-GFPlentiviral plasmid for expression of human CD24
205457TRE-UBC-hCD46-T2A-GFPlentiviral plasmid for expression of human CD46
205458TRE-UBC-hCD47-T2A-GFPlentiviral plasmid for expression of human CD47
205459TRE-UBC-hCD55-T2A-GFPlentiviral plasmid for expression of human CD55
205460TRE-UBC-hCD59-T2A-GFPlentiviral plasmid for expression of human CD59
205461TRE-UBC-HLA-E SCT-T2A-GFPlentiviral plasmid for expression of HLA-E SCT
205462TRE-UBC-HLA-G SCT-T2A-GFPlentiviral plasmid for expression of HLA-G SCT

Antibodies from Article