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A modular chemigenetic calcium indicator enables in vivo functional imaging with near-infrared light.

Farrants H, Shuai Y, Lemon WC, Hernandez CM, Yang S, Patel R, Qiao G, Frei MS, Grimm JB, Hanson TL, Tomaska F, Turner GC, Stringer C, Keller PJ, Beyene AG, Chen Y, Liang Y, Lavis LD, Schreiter ER
bioRxiv. 2023 Jul 19:2023.07.18.549527. doi: 10.1101/2023.07.18.549527. Preprint. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
205303pRSET-WHaloCaMP1a-EGFPExpression of WHaloCaMP1a-EGFP fusion in E. coli
205304pRSET-WHaloCaMP1aExpression of WHaloCaMP1a in E. coli
205305pRSET-WHaloCaMP1b-EGFPExpression of WHaloCaMP1b-EGFP fusion in E. coli
205306pRSET-WHaloCaMP-eNOSpep-EGFPExpression of WHaloCaMP-eNOSpep-EGFP fusion in E. coli
205307pAAV-synapsin-WHaloCaMP1a-EGFPProduction of AAV particles encoding WHaloCaMP1a-EGFP from the synapsin promoter
205308pAAV-synapsin-WHaloCaMP1aProduction of AAV particles encoding WHaloCaMP1a from the synapsin promoter
205309pAAV-CaMKII-WHaloCaMP1a-EGFPProduction of AAV particles encoding WHaloCaMP1a-EGFP from the CaMKII promoter
205310pAAV-CaMKII-WHaloCaMP1aProduction of AAV particles encoding WHaloCaMP1a from the CaMKII promoter
205311pTol2-elavl3-WHaloCaMP1a-EGFPExpression of WHaloCaMP1a-EGFP in zebrafish from the elavl3 promoter (pan-neuronal expression)
205312pTol2-elavl3-WHaloCaMP1aExpression of WHaloCaMP1a in zebrafish from the elavl3 promoter (pan-neuronal expression)
205313pCAG-WHaloCaMP1a-EGFPExpression of WHaloCaMP1a-EGFP in mammalian cells from the CAG promoter
205314p10xUAS-WHaloCaMP1a-EGFPExpression of WHaloCaMP1a-EGFP in Drosophila using GAL4 driver

Antibodies from Article