Single genomic enhancers drive experience-dependent GABAergic plasticity to maintain sensory processing in the adult cortex.
Roethler O, Zohar E, Cohen-Kashi Malina K, Bitan L, Gabel HW, Spiegel I
Neuron. 2023 Jun 20:S0896-6273(23)00399-9. doi: 10.1016/j.neuron.2023.05.026.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 203830 | pGL4.11_Nued2_E1 | Luciferase reporter plasmid for Igf1 enhancer 1 |
| 203831 | pGL4.11_Nued2_E2 | Luciferase reporter plasmid for Igf1 enhancer 2 |
| 203832 | pGL4.11_Nued2_E3 | Luciferase reporter plasmid for Igf1 enhancer 3 |
| 203833 | pGL4.11_Nued2_E4 | Luciferase reporter plasmid for Igf1 enhancer 4 |
| 203834 | pGL4.11_Nued2_E5 | Luciferase reporter plasmid for Igf1 enhancer 5 |
| 203835 | pGL4.11_Nued2_E1_dAP1 | Luciferase reporter plasmid for Igf1 enhancer 1 containing only the downstream AP1 site |
| 203836 | pGL4.11_Nued2_E1_dAP2 | Luciferase reporter plasmid for Igf1 enhancer 1 containing only the upstream AP1 site |
| 203837 | pGL4.11_Nued2_E1_dAP1-2 | Luciferase reporter plasmid for Igf1 enhancer 1 with both AP-1 sites knocked out |
| 203838 | pAAV-Syn1-fDIO-mScarlet-KASH-2a-Cre | Flp-dependent Cre and mScarlet-KASH expression |
| 203839 | pAAV-Syn1-fDIO-mScarlet-KASH-2a-dCre | Flp-dependent enzymatically inactive Cre and mScarlet-KASH expression |
| 203840 | pAAV-hSyn-Kir2.1WT-2A-mScarlet-KASH | Kir2.1 expression for silencing neurons and mScarlet-KASH expression |
| 203841 | pAAV-hSyn-Kir2.1Mut-2A-mScarlet-KASH | non-functional Kir2.1(mutant) expression as a control for the silencing of neurons with Kir2.1 and mScarlet-KASH expression |
| 203842 | pAAV-Ef1a-fDIO-eGFP-2A-Cre-WPRE | Flp-dependent Cre and EGFP expression |
| 203843 | pAAV-Ef1a-fDIO-eGFP-WPRE | Flp-dependent EGFP expression |
| 203844 | pAAV-Ef1a-fDIO-mRuby3-2A-Cre | Flp-dependent Cre and mRuby3 expression |
| 203845 | pAAV-Ef1a-fDIO-mRuby3-2A-dCre | Flp-dependent enzymatically inactive Cre and mRuby3 expression |