Evolutionary mining and functional characterization of TnpB nucleases identify efficient miniature genome editors.
Xiang G, Li Y, Sun J, Huo Y, Cao S, Cao Y, Guo Y, Yang L, Cai Y, Zhang YE, Wang H
Nat Biotechnol. 2023 Jun 29. doi: 10.1038/s41587-023-01857-x.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 205316 | pX601-AAV-CMV-NLS-ISAam1-NLS-3xHA-bGHpA;U6-BbsI-reRNA | All-in-one AAV vector expressing ISAam1 TnpB and its reRNA |
| 205317 | pX601-AAV-CMV-NLS-ISYmu1-NLS-3xHA-bGHpA;U6-BsaI-reRNA | All-in-one AAV vector expressing ISYmu1 TnpB and its reRNA |
| 205318 | pX601-AAV-CMV-NLS-ISDge10-NLS-3xHA-bGHpA;U6-BsaI-reRNA | All-in-one AAV vector expressing ISDge10 TnpB and its reRNA |
| 205319 | pX601-AAV-CMV-NLS-ISTfu1-NLS-3xHA-bGHpA;U6-BbsI-reRNA | All-in-one AAV vector expressing ISTfu1 TnpB and its reRNA |
| 205320 | pX601-AAV-CMV-NLS-ISAba30-NLS-3xHA-bGHpA;U6-BbsI-reRNA | All-in-one AAV vector expressing ISAba30 TnpB and its reRNA |
| 205321 | pBad33_IS605_reRNA | Bacterial expression of IS605 TnpB and its reRNA |
| 205322 | pBad33_ISAam1_reRNA | Bacterial expression of ISAam1 TnpB and its reRNA |
| 205323 | pBad33_ISAba30_reRNA | Bacterial expression of ISAba30 TnpB and its reRNA |
| 205324 | pBad33_ISAeme8_reRNA | Bacterial expression of ISAeme8 TnpB and its reRNA |
| 205325 | pBad33_ISAepa1_reRNA | Bacterial expression of ISAepa1 TnpB and its reRNA |
| 205326 | pBad33_ISAs26_reRNA | Bacterial expression of ISAs26 TnpB and its reRNA |
| 205327 | pBad33_ISBce3_reRNA | Bacterial expression of ISBce3 TnpB and its reRNA |
| 205328 | pBad33_ISBth16_reRNA | Bacterial expression of ISBth16 TnpB and its reRNA |
| 205329 | pBad33_ISCath1_reRNA | Bacterial expression of ISCath1 TnpB and its reRNA |
| 205330 | pBad33_ISCbt1_reRNA | Bacterial expression of ISCbt1 TnpB and its reRNA |
| 205331 | pBad33_ISCbt2_reRNA | Bacterial expression of ISCbt2 TnpB and its reRNA |
| 205332 | pBad33_ISCco1_reRNA | Bacterial expression of ISCco1 TnpB and its reRNA |
| 205333 | pBad33_ISClsp11_reRNA | Bacterial expression of ISClsp11 TnpB and its reRNA |
| 205334 | pBad33_ISClte2_reRNA | Bacterial expression of ISClte2 TnpB and its reRNA |
| 205335 | pBad33_ISCpe2_reRNA | Bacterial expression of ISCpe2 TnpB and its reRNA |
| 205336 | pBad33_ISCvi1_reRNA | Bacterial expression of ISCvi1 TnpB and its reRNA |
| 205337 | pBad33_ISCvi2_reRNA | Bacterial expression of ISCvi2 TnpB and its reRNA |
| 205338 | pBad33_ISCysp13_reRNA | Bacterial expression of ISCysp13 TnpB and its reRNA |
| 205339 | pBad33_ISCytsp1_reRNA | Bacterial expression of ISCytsp1 TnpB and its reRNA |
| 205340 | pBad33_ISDge10_reRNA | Bacterial expression of ISDge10 TnpB and its reRNA |
| 205341 | pBad33_ISEc41_reRNA | Bacterial expression of ISEc41 TnpB and its reRNA |
| 205342 | pBad33_ISEc44_reRNA | Bacterial expression of ISEc44 TnpB and its reRNA |
| 205343 | pBad33_ISEc46_reRNA | Bacterial expression of ISEc46 TnpB and its reRNA |
| 205344 | pBad33_ISEfa4_reRNA | Bacterial expression of ISEfa4 TnpB and its reRNA |
| 205345 | pBad33_ISHahl1_reRNA | Bacterial expression of ISHahl1 TnpB and its reRNA |
| 205346 | pBad33_ISKpn69_reRNA | Bacterial expression of ISKpb69 TnpB and its reRNA |
| 205347 | pBad33_ISMac7_reRNA | Bacterial expression of ISMac7 TnpB and its reRNA |
| 205348 | pBad33_ISMma22_reRNA | Bacterial expression of ISMma22 TnpB and its reRNA |
| 205349 | pBad33_ISMmu1_reRNA | Bacterial expression of ISMmu1 TnpB and its reRNA |
| 205350 | pBad33_ISNsp2_reRNA | Bacterial expression of ISNsp2 TnpB and its reRNA |
| 205351 | pBad33_ISNsp3_reRNA | Bacterial expression of ISNsp3 TnpB and its reRNA |
| 205352 | pBad33_ISRor9_reRNA | Bacterial expression of ISRor9 TnpB and its reRNA |
| 205353 | pBad33_ISSen6_reRNA | Bacterial expression of ISSen6 TnpB and its reRNA |
| 205355 | pBad33_ISTfu1_reRNA | Bacterial expression of ISTfu1 TnpB and its reRNA |
| 205356 | pBad33_ISTva1_reRNA | Bacterial expression of ISTva1 TnpB and its reRNA |
| 205357 | pBad33_ISVgsp2_reRNA | Bacterial expression of ISVgsp2 TnpB and its reRNA |
| 205358 | pBad33_ISYmu1_reRNA | Bacterial expression of ISYmu1 TnpB and its reRNA |