FREEDA: An automated computational pipeline guides experimental testing of protein innovation.
Dudka D, Akins RB, Lampson MA
J Cell Biol. 2023 Sep 4;222(9):e202212084. doi: 10.1083/jcb.202212084. Epub 2023 Jun 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 205178 | pGEMHE-MmCENP-O-GFP | Expresses mouse CENP-O C-term tagged with GFP under T7 promoter - designed for in vitro transcription |
| 205179 | pGEMHE-RnCENP-O-GFP | Expresses rat CENP-O C-term tagged with GFP under T7 promoter - designed for in vitro transcription |
| 205181 | pGEMHE-RnCENP-O-11NS-GFP | Expresses rat CENP-O with 11 mouse-specific point mutations in residues under positive selection; C-term tagged with GFP under T7 promoter - designed for in vitro transcription |
| 205182 | pGEMHE-RnCENP-O-80-GFP | Expresses chimeric rat CENP-O with mouse CENP-O N-terminus; C-term tagged with GFP under T7 promoter - designed for in vitro transcription |
| 205183 | pGEMHE-RnCENP-O-203-GFP | Expresses chimeric rat CENP-O with mouse CENP-O middle region including central RWD domain; C-term tagged with GFP under T7 promoter - designed for in vitro transcription |
| 205184 | pGEMHE-RnCENP-O-298-GFP | Expresses chimeric rat CENP-O with mouse CENP-O C-terminal RWD domain; C-term tagged with GFP under T7 promoter - designed for in vitro transcription |
| 205185 | pGEMHE-MmCENP-O-R298-GFP | Expresses chimeric mouse CENP-O with rat CENP-O C-terminal RWD domain; C-term tagged with GFP under T7 promoter - designed for in vitro transcription |
| 205742 | pGEMHE-RnCENP-O-298-5NS-GFP | Expresses chimeric rat CENP-O with mouse CENP-O C-terminal RWD domain and 5 mouse-specific point mutations; C-term tagged with GFP under T7 promoter - designed for in vitro transcription |