A PI(3,5)P2 reporter reveals PIKfyve activity and dynamics on macropinosomes and phagosomes.
Vines JH, Maib H, Buckley CM, Gueho A, Zhu Z, Soldati T, Murray DH, King JS
J Cell Biol. 2023 Sep 4;222(9):e202209077. doi: 10.1083/jcb.202209077. Epub 2023 Jun 29.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 205128 | pJSK659 | Expression of GFP-SnxA in mammalian cells |
| 205129 | pJSK663 | Expression of GFP-1xPX (from SnxA) in mammalian cells |
| 205130 | pJSK664 | Expression of GFP-2xPX (from SnxA) in mammalian cells |
| 205131 | pJSK418 | Expression of GFP-2xFYVE in Dictyostelium |
| 205132 | pJV0010 | Expression of 1xPX-GFP in Dictyostelium |
| 205133 | pJV0016 | Expression of 2xPX-GFP in Dictyostelium |
| 205134 | pJSK619 | Expression of SnxA-GFP in Dictyostelium |
| 205136 | pCB40 | Integrating expression of SnxA-GFP in Dictyostelium |
| 205137 | pCB128 | Expression of mCherry-2xFYVE in Dictyostelium |
| 205138 | pJV0042 | Expression of 6xHis-EGFP-SnxA in bacteria |
| 205139 | pJV0047 | Expression of 6xHis-EGFP-2xPX in bacteria |