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Jeremy Baskin Lab: A phosphorylation-controlled switch confers cell cycle-dependent protein relocalization

Unpublished

Plasmids from Article

ID Plasmid Purpose
205232MARSExpresses PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-i in mammalian cells
2052332xMARSExpresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-iin mammalian cells
205234MARS-nGFPExpresses PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells
2052352xMARS-nGFPExpresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells
205236pCDNA3-MARS-p85(iSH2)Expresses PLEKAH5 aa 143-271 (K163A and R164A) fused to the iSH2 domain of mouse p85beta
205237pCDNA3-2xMARS-PLDExpresses two repeats of PLEKAH5 aa 143-271 (K163A and R164A) fused to phospholipase D from Streptomyces sp. PMF
205238pCDH-2xMARS-nGFP-puroLentiviral vector encoding two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody
205239pSBtet-2xMARS-nGFP-puroDOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon system

Antibodies from Article