In vivo expression vector derived from anhydrobiotic tardigrade genome enables live imaging in Eutardigrada.
Tanaka S, Aoki K, Arakawa K
Proc Natl Acad Sci U S A. 2023 Jan 31;120(5):e2216739120. doi: 10.1073/pnas.2216739120. Epub 2023 Jan 24.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 205001 | pRvACT-mEGFP-NLS | The vector contains 1kbp of the upstream and downstream regions of the actin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between. |
| 205002 | pRvACT-mCherry | The vector contains 1kbp of the upstream and downstream regions of the actin gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between. |
| 205003 | pHeACT-mEGFP-NLS | The vector contains 1kbp of the upstream and downstream regions of the actin gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between. |
| 205004 | pRvCAHS3-mEGFP-NLS | The vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between. |
| 205005 | pRvSAHS1-mEGFP-NLS | The vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between. |
| 205006 | pRvCAHS3-CAHS3-mEGFP | The vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the RvCAHS3-mEGFP gene positioned in between. |
| 205007 | pRvSAHS1-SAHS1-mEGFP | The vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the RvSAHS1-mEGFP gene positioned in between. |
| 205008 | pRvCAHS3-mEGFP | The vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene positioned in between. |
| 205009 | pRvSAHS1-mCherry-NLS | The vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between. |
| 205010 | pRvUBC-mEGFP-NLS | The vector contains 1kbp of the upstream and downstream regions of the ubiquitin c gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between. |
| 205011 | pRvTUB-mEGFP-NLS | The vector contains 1kbp of the upstream and downstream regions of the tubulin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between. |
| 205012 | pHeEf1a-mEGFP-NLS | The vector contains 1kbp of the upstream and downstream regions of the ef1a gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between. |
| 205013 | pRvCAHS1-mEGFP-NLS | The vector contains 1kbp of the upstream and downstream regions of the cahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between. |
| 205014 | pHeCAHS-mEGFP-NLS | The vector contains 1kbp of the upstream and downstream regions of the chas gene (BV898_02951, CAHS 86272) from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between. |