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Discovery of deaminase functions by structure-based protein clustering.

Huang J, Lin Q, Fei H, He Z, Xu H, Li Y, Qu K, Han P, Gao Q, Li B, Liu G, Zhang L, Hu J, Zhang R, Zuo E, Luo Y, Ran Y, Qiu JL, Zhao KT, Gao C
Cell. 2023 Jul 20;186(15):3182-3195.e14. doi: 10.1016/j.cell.2023.05.041. Epub 2023 Jun 27. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
204849p2T-CMV-miniSdd3-BE4max-BlastRFor cytosine base editing using miniSdd3 in mammalian cells
204850p2T-CMV-miniSdd6-BE4max-BlastRFor cytosine base editing using miniSdd6 in mammalian cells
204851p2T-CMV-miniSdd7-BE4max-BlastRFor cytosine base editing using miniSdd7 in mammalian cells
204852pAAV-EFS-SaKKH-spA-miniU6-miniSdd6-MmHPD-T2AAV vector for cytosine base editing using miniSdd6 at HPD-T2 target site in mouse
204853pCMV-TALE-L-JAK2-Ddd9-NFor DdCBE editing using Ddd9 at JAK2 site in human cells
204854pCMV-TALE-R-JAK2-Ddd9-CFor DdCBE editing using Ddd9 at JAK2 site in human cells
204855pE-35S-miniSdd7-nCas9-PBE-GmPPO2For cytosine base editing using miniSdd7 at PPO2 site in soybean (Agrobacterium-mediated transformation)
204856pH-Ubi-miniSdd7-nCas9-PBEFor cytosine base editing using miniSdd7 in monocotyledon plants (Agrobacterium-mediated transformation)
204857pnCas9-miniSdd3-PBEFor cytosine base editing using miniSdd3 in monocotyledon plants (transient transformation)
204858pnCas9-miniSdd6-PBEFor cytosine base editing using miniSdd6 in monocotyledon plants (transient transformation)
204859pnCas9-miniSdd7-PBEFor cytosine base editing using miniSdd7 in monocotyledon plants (transient transformation)

Antibodies from Article