Programmable protein expression using a genetically encoded m(6)A sensor.
Marayati BF, Thompson MG, Holley CL, Horner SM, Meyer KD
Nat Biotechnol. 2024 Jan 2. doi: 10.1038/s41587-023-01978-3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 204765 | pGEMS-II | GEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577) |
| 204766 | pGEMS-II-PEST | GEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST) |
| 204767 | pGEMS-II-dCas13-APO1-YTH | GEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed) |
| 204768 | pU6-PspCas13b-GEMSgRNA2 | pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence. |