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Addgene

A living material platform for the biomineralization of biosilica.

Kirpat Konak BM, Bakar ME, Ahan RE, Ozyurek EU, Dokmeci S, Safak Seker UO
Mater Today Bio. 2022 Oct 10;17:100461. doi: 10.1016/j.mtbio.2022.100461. eCollection 2022 Dec 15. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
225139pET22b Ag43 160N 6H sfGFP-R5 AmpRRecombinant Ag43 protein fused to silaffin-R5 and sfGFP. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.
225141pET22b Ag43 160N 6H mCherry-R5 AmpRRecombinant Ag43 protein fused to silaffin-R5 and mCherry. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.

Antibodies from Article