An engineered hypercompact CRISPR-Cas12f system with boosted gene-editing activity.
Wu T, Liu C, Zou S, Lyu R, Yang B, Yan H, Zhao M, Tang W
Nat Chem Biol. 2023 Jul 3. doi: 10.1038/s41589-023-01380-9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 204635 | CMV-AsCas12f-WT | Expression of wild-type AsCas12f in mammalian cells |
| 204636 | CMV-AsCas12f-v4.1 | Expression of AsCas12f v4.1 in mammalian cells |
| 204637 | CMV-enAsCas12f | Expression of enAsCas12f in mammalian cells |
| 204638 | U6-AsCas12f-full-length-sgRNA | Expression of full-length AsCas12f sgRNA in mammalian cells |
| 204639 | U6-AsCas12f-sgRNA-v2 | Expression of trancated AsCas12f sgRNA in mammalian cells |
| 204640 | pET47b-T7-AsCas12f-WT | Expression of wild-type AsCas12f in E. coli |
| 204641 | pET47b-T7-dAsCas12f | Expression of an inactive AsCas12f in E. coli |
| 204642 | pET47b-T7-enAsCas12f | Expression of enAsCas12f in E. coli |