Dual CRISPR-Cas3 system for inducing multi-exon skipping in DMD patient-derived iPSCs.
Kita Y, Okuzaki Y, Naoe Y, Lee J, Bang U, Okawa N, Ichiki A, Jonouchi T, Sakurai H, Kojima Y, Hotta A
Stem Cell Reports. 2023 Sep 12;18(9):1753-1765. doi: 10.1016/j.stemcr.2023.07.007. Epub 2023 Aug 24.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 204620 | pPV-C1-crRNA(DMD#20_DMD#23)-EF1a-BA | Expression vector of crRNA (DMD#20) targeting the dystrophin intron 44 just upstream of exon 45, and crRNA(DMD#23)targeting the dystrophin intron 55 just downstream of exon 55. Blasticidin selectable. |
| 204621 | pPV-EF1a-EGxxFP(DMDex45_10)-iP-A | Single-strand annealing (SSA) vector for monitoring crRNA(DMDex45)'s activity. DMD intron 44 region (upstream of exon 45) |
| 204622 | pPV-EF1α-mRxxFP1(DMDex55_10)-iP-A | Single-strand annealing (SSA) vector for monitoring crRNA(DMDex55)'s activity. DMD intron 55 region (downstream of exon 55). Puromycin selectable. |
| 204623 | pPV-C1-crRNA-cloning-EF1a-BA | piggyBac vector for crRNA cloning and expression. Blasticidin selectable. |
| 204624 | pPV-EF1a-EGxxFP-iP-A | Cloning vector for SSA vector (EGxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestion |
| 204625 | pPV-EF1a-mRxxFP1(AfeI)-iP-A | Cloning vector for SSA vector (mRxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestion |