Dual CRISPR-Cas3 system for inducing multi-exon skipping in DMD patient-derived iPSCs.

Kita Y, Okuzaki Y, Naoe Y, Lee J, Bang U, Okawa N, Ichiki A, Jonouchi T, Sakurai H, Kojima Y, Hotta A
Stem Cell Reports. 2023 Sep 12;18(9):1753-1765. doi: 10.1016/j.stemcr.2023.07.007. Epub 2023 Aug 24. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
204625	pPV-EF1a-mRxxFP1(AfeI)-iP-A	Cloning vector for SSA vector (mRxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestion
204624	pPV-EF1a-EGxxFP-iP-A	Cloning vector for SSA vector (EGxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestion
204623	pPV-C1-crRNA-cloning-EF1a-BA	piggyBac vector for crRNA cloning and expression. Blasticidin selectable.
204622	pPV-EF1α-mRxxFP1(DMDex55_10)-iP-A	Single-strand annealing (SSA) vector for monitoring crRNA(DMDex55)'s activity. DMD intron 55 region (downstream of exon 55). Puromycin selectable.
204621	pPV-EF1a-EGxxFP(DMDex45_10)-iP-A	Single-strand annealing (SSA) vector for monitoring crRNA(DMDex45)'s activity. DMD intron 44 region (upstream of exon 45)
204620	pPV-C1-crRNA(DMD#20_DMD#23)-EF1a-BA	Expression vector of crRNA (DMD#20) targeting the dystrophin intron 44 just upstream of exon 45, and crRNA(DMD#23)targeting the dystrophin intron 55 just downstream of exon 55. Blasticidin selectable.