Virus-assisted directed evolution of enhanced suppressor tRNAs in mammalian cells.
Jewel D, Kelemen RE, Huang RL, Zhu Z, Sundaresh B, Cao X, Malley K, Huang Z, Pasha M, Anthony J, van Opijnen T, Chatterjee A
Nat Methods. 2023 Jan;20(1):95-103. doi: 10.1038/s41592-022-01706-w. Epub 2022 Dec 22.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 204868 | pAAV-ITR-PytR-mCherry | Expresses pyrrolysyl tRNA and a wild-type mCherry reporter; can be packaged into AAV |
| 204869 | pAAV-ITR-PytR(A2.1)-mCherry | Expresses pyrrolysyl tRNA variant "A2.1" and a wild-type mCherry reporter; can be packaged into AAV |
| 217360 | pAcBac1-GFP(39TAG) | Expression of reporter EGFP with a TAG stop codon at 39 aa position; can be packaged into bacmid |
| 217361 | pIDTSmart-MbPylRS | Expresses Methanosarcina barkeri aminoacyl-tRNA synthetases; can be packaged into bacmid |
| 217362 | pAAV-ITR-EcYtR-mCherry | Expresses E. coli tyrosine tRNA and a wild-type mCherry reporter; can be packaged into AAV |
| 217363 | pAAV-ITR-EcYtR(NGS-6)-mCherry | Expresses E. coli tyrosine tRNA variant "NGS-6" and a wild-type mCherry reporter; can be packaged into AAV |