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In vitro analyses of the production and activity of secondary small interfering RNAs in C. elegans.

Aoki K, Moriguchi H, Yoshioka T, Okawa K, Tabara H
EMBO J. 2007 Dec 12. 26(24):5007-19. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
204509pHIT198pET-derived vector for expression of proteins N-terminally tagged with 6xHis and maltose binding protein (multi-cloning sites: NdeI, NotI, SpeI, SalI, PstI, SgfI, and XhoI)
204510pHIT179Bacterial expression of CSR-1a with 6xHis and maltose binding protein tags for in vitro assays
204511pHIT182Bacterial expression of catalytically defective CSR-1a with 6xHis and maltose binding protein tags for in vitro assays

Antibodies from Article