A small RNA system ensures accurate homologous pairing and unpaired silencing of meiotic chromosomes.
Tabara H, Mitani S, Mochizuki M, Kohara Y, Nagata K
EMBO J. 2023 Jun 1;42(11):e105002. doi: 10.15252/embj.2020105002. Epub 2023 Apr 20.
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| ID | Plasmid | Purpose |
|---|---|---|
| 204505 | pHIT346 | 5S rDNA used as a probe for chromosome FISH in nematode |
| 204506 | pHIT353 | In vitro transcription of RNA probes for in situ hybridization of CeRep55 lncRNAs in nematode (after linearization with NotI and BglI) |
| 204507 | pHIT246 | csr-1 construct tagged with 2xFLAG for MosSCI integration at locus ttTi5605 on Chr II in nematode |
| 204508 | pHIT318 | rpl-21 promoter-driven C04F12.1 construct tagged with 2xHA for MosSCI integration at locus ttTi5605 on Chr II in nematode |
| 204512 | pHIT203 | Bacterial expression of C04F12.1 with 6xHis and maltose binding protein tags for in vitro assays |
| 204513 | pHIT186 | Bacterial expression of CSR-1 PAZ domain with 6xHis and maltose binding protein tags for use as an antigen |
| 204514 | pHIT342 | Bacterial expression of SYP-1 N-terminal fragment with 6xHis and maltose binding protein tags for use as an antigen |
| 204515 | pHIT348 | Bacterial expression of CEC-5 C-terminal fragment with 6xHis and maltose binding protein tags for use as an antigen |
| 204516 | pHIT334 | Bacterial expression of COH-3 C-terminal fragment with 6xHis and maltose binding protein tags for use as an antigen |
| 204517 | pHIT320 | Bacterial expression of HIM-1 internal fragment with 6xHis and maltose binding protein tags for use as an antigen |
| 204518 | pHIT517 | Bacterial expression of CEC-4 with 6xHis and maltose binding protein tags for in vitro assays |
| 204519 | pHIT520 | Bacterial expression of CEC-8 N-terminal fragment with 6xHis and maltose binding protein tags for in vitro assays |
| 204520 | pHIT521 | Bacterial expression of CEC-5 N-terminal fragment with 6xHis and maltose binding protein tags for in vitro assays |
| 204522 | pHIT239 | In vitro transcription of Mos1 transposase mRNA with SL1 and poly(A) used for microinjection into nematode |