A metagenomic library cloning strategy that promotes high-level expression of captured genes to enable efficient functional screening.
Rich MH, Sharrock AV, Mulligan TS, Matthews F, Brown AS, Lee-Harwood HR, Williams EM, Copp JN, Little RF, Francis JJ, Horvat CN, Stevenson LJ, Owen JG, Saxena MT, Mumm JS, Ackerley DF
bioRxiv. 2023 Mar 25:2023.03.24.534183. doi: 10.1101/2023.03.24.534183. Preprint.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 204409 | pUCXMG | Expression plasmid for cloning of FatI digested metagenomic DNA into a compatible NcoI site |