Identifying LasR Quorum Sensors with Improved Signal Specificity by Mapping the Sequence-Function Landscape.
Zeng M, Sarker B, Rondthaler SN, Vu V, Andrews LB
ACS Synth Biol. 2024 Feb 16;13(2):568-589. doi: 10.1021/acssynbio.3c00543. Epub 2024 Jan 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 204463 | pMZ103-rbs1-m1 | This is a biosensor with improved specificity to 3OC12-HSL, containing LasR A127N mutation. |
| 204464 | pMZ103-rbs1-m2 | This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L128C mutation. |
| 204465 | pMZ103-rbs1-m3 | This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127S mutation. |
| 204466 | pMZ103-rbs1-m4 | This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127T mutation. |
| 204467 | pMZ103-rbs1-m5 | This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125F-A127G-S129N mutation. |
| 204468 | pMZ103-rbs1-m6 | This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-S129N-L130V mutation. |
| 204469 | pMZ103-rbs1-m7 | This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127T-L128M mutation. |
| 204470 | pMZ103-rbs1-m8 | This is a biosensor with decreased specificity to 3OC12-HSL and improved specificity to 3OHC14-HSL, containing LasR L128F-S129W mutation. |