The Janelia Atalanta plasmids provide a simple and efficient CRISPR/Cas9-mediated homology directed repair platform for Drosophila
Stern D, Kim E, Behrman E
bioRxiv 2023.06.17.545412
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 204289 | pJAT39 | For marking CRISPR HDR integrant with EGFP expressed in flight muscles. |
| 204290 | pJAT11 | For inserting a phiC31 attP site. HDR integrant is marked with removable ie1-DsRed cassette, which is expressed in abdomen and mouthparts. |
| 204291 | pJAT31 | For inserting a phiC31 attP site. HDR integrant is marked with ie1-DsRed, which is expressed in abdomen and mouthparts. Second U6 promoter allows use of two different gRNAs in two synthesized arms. |
| 204292 | pJAT7 | For inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope. |
| 204293 | pJAT10 | For marking CRISPR HDR integrant with DsRed expressed in flight muscles. |
| 204294 | pJAT30 | For marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs. |
| 204295 | pJAT77 | pJAT plasmid with multiple cloning site for introducing custom payload. No U6 promoter. Restriction sites present to introduce flanking U6 promoters. |
| 204296 | pJAT74 | pJAT plasmid with multiple cloning site for introducing custom payload. One U6 promoter. |
| 204297 | pJAT32 | pJAT plasmid with multiple cloning site for introducing custom payload. Two U6 promoters. |
| 204298 | pJAT17 | For introducing Cas9 under D. melanogaster nanos promoter. Markers with EYFP expressed in eyes. |
| 204299 | pJAT43 | For introducing heat shock inducble B3 recombinase. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts. |
| 204300 | pJAT56 | FExpress phiC31 integrase under heat shock control. Insert marked with 3XP3-EGFP, which expresses in eyes. |
| 204301 | pJAT62 | Express phiC31 integrase under heat shock control. Insert marked with ie1-EGFP, which expresses in abdomen and mouthparts. |
| 204303 | pJAT57 | Expresses Cas9 under UAS control. HDR integrant markers with DsRed expressed in flight muscles. |
| 204304 | pJAT58 | Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable 3XP3-DsRed cassette expressed in eyes. |
| 204305 | pJAT71 | Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable ie1XP3-DsRed cassette expressed in abdomen and mouthparts. |
| 204306 | pJAT75 | Flippase under heat shock control. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts |
| 204307 | pJAT18 | For generating targeting inversions. N-terminal split GFP, expressed in flight muscles. |
| 204308 | pJAT27 | For generating targeting inversions. C-terminal split GFP, expressed in flight muscles. |
| 204309 | pJAT21 | For generating targeting inversions. N-terminal split GFP, expressed in abdomen and mouthparts |
| 204310 | pJAT25 | For generating targeting inversions. C-terminal split GFP, expressed in abdomen and mouthparts. |
| 204312 | pJAT23 | For generating targeting inversions. C-terminal split GFP, expressed in eyes. |