Organelle and translocatable forms of glyoxysomal malate dehydrogenase. The effect of the N-terminal presequence.
Cox B, Chit MM, Weaver T, Gietl C, Bailey J, Bell E, Banaszak L
FEBS J. 2005 Feb;272(3):643-54. doi: 10.1111/j.1742-4658.2004.04475.x.
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PubMed
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Article
Please see Addgene's curated Malate Dehydrogenase CUREs Community (MCC) Collection page for additional information about these plasmids.
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 204246 | pQE60 Q58L wgMDH | Bacterial expression of Q58L wgMDH with IPTG induction - Subunit interface mutant |
| 204247 | pQE60 M66L wgMDH | Bacterial expression of M66L wgMDH with IPTG induction - Subunit interface mutant |
| 204248 | pQE60 D87L wgMDH | Bacterial expression of D87L wgMDH with IPTG induction - Subunit interface mutant |
| 204250 | pQE60 M91Q wgMDH | Bacterial expression of M91Q wgMDH with IPTG induction - Subunit interface mutant |
| 204252 | pQE60 G121A wgMDH | Bacterial expression of G121A wgMDH with IPTG induction - Active site loop mutant |
| 204253 | pQE60 G121F wgMDH | Bacterial expression of G121F wgMDH with IPTG induction - Active site loop mutant |
| 204254 | pQE60 P123A wgMDH | Bacterial expression of P123A wgMDH with IPTG induction - Active site loop mutant |
| 204255 | pQE60 P123F wgMDH | Bacterial expression of P123F wgMDH with IPTG induction - Active site loop mutant |
| 204256 | pQE60 P123G wgMDH | Bacterial expression of P123G wgMDH with IPTG induction - Active site loop mutant |
| 204257 | pQE60 R124A wgMDH | Bacterial expression of R124A wgMDH with IPTG induction - Active site mutant |
| 204258 | pQE60 R124K wgMDH | Bacterial expression of R124K wgMDH with IPTG induction - Active site mutant |
| 204259 | pQE60 R124Q wgMDH | Bacterial expression of R124Q wgMDH with IPTG induction - Active site mutant |
| 204260 | pQE60 P126G wgMDH | Bacterial expression of P126G wgMDH with IPTG induction - Active site loop mutant |
| 204261 | pQE60 P126R wgMDH | Bacterial expression of P126R wgMDH with IPTG induction - Active site loop mutant |
| 204263 | pQE60 G127A wgMDH | Bacterial expression of G127A wgMDH with IPTG induction - Active site loop mutant |
| 204264 | pQE60 G127F wgMDH | Bacterial expression of G127F wgMDH with IPTG induction - Active site loop mutant |
| 204265 | pQE60 G127P wgMDH | Bacterial expression of G127P wgMDH with IPTG induction - Active site loop mutant |
| 204266 | pQE60 R130A wgMDH | Bacterial expression of R130A wgMDH with IPTG induction - Active site mutant |
| 204267 | pQE60 R130E wgMDH | Bacterial expression of R130E wgMDH with IPTG induction - Active site mutant |
| 204268 | pQE60 R130Q wgMDH | Bacterial expression of R130Q wgMDH with IPTG induction - Active site mutant |
| 204269 | pQE60 D193E wgMDH | Bacterial expression of D193E wgMDH with IPTG induction - Active site mutant |
| 204271 | pQE60 R196A wgMDH | Bacterial expression of R196A wgMDH with IPTG induction - Active site mutant |
| 204272 | pQE60 R196E wgMDH | Bacterial expression of R196E wgMDH with IPTG induction - Active site mutant |
| 204273 | pQE60 R196M wgMDH | Bacterial expression of R196M wgMDH with IPTG induction - Active site mutant |