Deciphering RNA splicing logic with interpretable machine learning.

Liao SE, Sudarshan M, Regev O
Proc Natl Acad Sci U S A. 2023 Oct 10;120(41):e2221165120. doi: 10.1073/pnas.2221165120. Epub 2023 Oct 5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
204187	pRegev_ExonSkip_Backbone	Backbone for cloning splicing library with truncated CAG promoter and first half of beta globin minigene reporter (exon 1 and intron 1).
204186	pRegev_ExonSkip_LandingPad	Backbone for cloning individual exon sequences into beta globin minigene splicing reporter.
204185	pRegev_D4.34C>G	pRegev_D4 with single C>G mutation.
204184	pRegev_D4	Single reporter with long guanine depleted (G-poor) region from splicing library.
204183	pRegev_D3.48C>G	pRegev_D3 with single C>G mutation.
204182	pRegev_D3	Single reporter with long guanine depleted (G-poor) region from splicing library.
204181	pRegev_D2.30C>G	pRegev_D2 with single C>G mutation.
204180	pRegev_D2	Single reporter with long guanine depleted (G-poor) region from splicing library.
204179	pRegev_D1.61C>G	pRegev_D1 with single C>G mutation.
204178	pRegev_D1	Single reporter with long guanine depleted (G-poor) region from splicing library.