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Development and characterization of novel jGCaMP8f calcium sensor variants with improved kinetics and fluorescence response range.

Tran O, Hughes HJ, Carter T, Torok K
Front Cell Neurosci. 2023 May 18;17:1155406. doi: 10.3389/fncel.2023.1155406. eCollection 2023. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
204138pET30b jGCaMP8f L27A Production of recombinant calcium sensor jGCaMP8f L27A fast decay variant in E.coli
204139pET30b jGCaMP8f F366HProduction of recombinant calcium sensor jGCaMP8f F366H high dynamic range variant in E.coli
204140pGP-CMV-jGCaMP8f L27AFluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with L27A mutation
204141pGP-CMV-jGCaMP8f F366HFluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutation

Antibodies from Article