Light-switchable transcription factors obtained by direct screening in mammalian cells.
Zhu L, McNamara HM, Toettcher JE
Nat Commun. 2023 Jun 2;14(1):3185. doi: 10.1038/s41467-023-38993-6.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 203907 | pSFFV_Gal4LOV[SK22]-VP64-IRES-mCherry (pLZA140) | Encodes LightsOut (AsLOV2(408-543) inserted in Gal4 SK22) transcription factor in mammalian cells |
| 203908 | pHR_Gal4UAS_dGFP_PGK_iRFP (pLZA042) | Encodes dGFP downstream UAS promoter that's responsive to Gal4-VP64 activity in mammalian cells |
| 203909 | pSFFV_Gal4-VP64-IRES-mCherry (pLZA139) | Encodes wild type Gal4-VP64 transcription factor in mammalian cells |
| 203910 | pEF-1α_ZF9LOV[C1]-VP64 (pLZA125) | Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C1 position in mammalian cells |
| 203911 | pEF-1α_ZF9LOV[C2]-VP64 (pLZA126) | Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C2 position in mammalian cells |