The role of the Golden2-like (GLK) transcription factor in regulating terpenoid indole alkaloid biosynthesis in Catharanthus roseus.
Cole-Osborn LF, McCallan SA, Prifti O, Abu R, Sjoelund V, Lee-Parsons CWT
Plant Cell Rep. 2024 May 14;43(6):141. doi: 10.1007/s00299-024-03208-9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 203886 | pUN381 - pTRV2-CHLH | Viral silencing plasmid targeting the CHLH gene from C. roseus. For use as a positive control for VIGS experiments. |
| 203888 | pUN378 - pTRV2-CrGLK | Viral silencing plasmid targeting the CrGLK gene from C. roseus. |
| 203889 | pSB278 - pL0_pT16H2 (pro + 5U) | T16H2 promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid. |
| 203890 | pUN1213 - pL0_p16OMT (pro + 5U) | 16OMT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid. |
| 203891 | pUN1243 - pL0_pT3O (pro + 5U) | T3O promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid. |
| 203892 | pUN1224 - pL0_pT3R (pro + 5U) | T3R promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid. |
| 203893 | pUN1214 - pL0_pNMT (pro + 5U) | NMT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid. |
| 203894 | pUN1198 - pL0_pD4H (pro + 5U) | D4H promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid. |
| 203895 | pUN1215 - pL0_pDAT (pro + 5U) | DAT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid. |
| 203903 | pSB55 - pL0_CrGLK (CDS) | MoClo compatible L0 plasmid containing the CrGLK coding sequence |
| 203904 | pLC045 - pL0_CrGLK (CDS1ns) | MoClo compatible L0 plasmid containing the CrGLK coding sequence, lacking a stop codon to allow for C-terminal fusions. |
| 203905 | pLC025 - pL0_StopCodon (CT) | MoClo compatible L0 plasmid containing a stop codon. To allow for proper fusion with CDS1ns L0 plasmids, this plasmid adds an additional glycine and serine to the C-terminal end of a CDS and then adds a stop codon to terminate translation. |
| 203906 | pLC047 - pL2_p2x35s-CrGLK-tMAS | Overexpression of the CrGLK coding sequence from C. roseus in a plant binary vector for Agrobacterium-mediated transformation. |