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RNA-Guided DNA Transposition in Corynebacterium glutamicum and Bacillus subtilis.

Yang S, Zhu J, Zhou X, Zhang J, Li Q, Bian F, Zhu J, Yan T, Wang X, Zhang Y, Yang J, Jiang Y, Yang S
ACS Synth Biol. 2023 Jul 21;12(7):2198-2202. doi: 10.1021/acssynbio.3c00193. Epub 2023 Jun 22. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
203812pBsVchCASTExpression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli
203813pBsVchCAST-pyrEExpression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli
203814pBsVchDonorDNA donor for transposition
203815pCgVchcrRNAExpression of CRISPR RNA, shuttle vector for Corynebacterium glutamicum / E. coli
203816pCgVchCAST-crtYfExpression of CRISPR-associated transposases, shuttle vector for Corynebacterium glutamicum / E. coli
203817pCgVchDonorDNA donor for transposition, shuttle vector for Corynebacterium glutamicum / E. coli

Antibodies from Article