Cas12n nucleases, early evolutionary intermediates of type V CRISPR, comprise a distinct family of miniature genome editors.
Chen W, Ma J, Wu Z, Wang Z, Zhang H, Fu W, Pan D, Shi J, Ji Q
Mol Cell. 2023 Jun 27:S1097-2765(23)00463-X. doi: 10.1016/j.molcel.2023.06.014.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 203806 | p15a_AcCas12n | p15a-based plasmid containing a rpsL driven AcCas12n cassette and the araBAD-driven lambda-red recombinase cassette. Apramycin resistance. |
| 203807 | pSGKP_AcsgRNA | pSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance. |
| 203808 | pET28a_SUMO_AcCas12n | pET28a-based plasmid containing a T7-driven His6-SUMO-HRV3c-AcCas12n cassette. Kanamycin resistance. |
| 203809 | pAcCas12nHS_ABE_D3_empty | Plasmid containing a CMV-driven NLS-dAcCas12n(D240)-ABE-Design3-NLS cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion). |
| 203810 | pAcCas12nHS_RV6_PT1_empty | Plasmid containing a CMV-driven NLS-AcCas12n-PT1-NLS(human-codonoptimized)cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion). Carbenicillin resistance. |
| 203811 | pAcCas12nHS_RV6_empty | Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance. |