A nucleotide binding-independent role for gamma-tubulin in microtubule capping and cell division.
Berman AY, Wieczorek M, Aher A, Olinares PDB, Chait BT, Kapoor TM
J Cell Biol. 2023 Mar 6;222(3):e202204102. doi: 10.1083/jcb.202204102. Epub 2023 Jan 25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 205602 | pSuperior.retro.puro-sh-γ-tubulin | Tet-inducible expression of shRNA targeting gamma-tubulin in human cells, retroviral |
| 205603 | pCDNA5/FRT/TO–γ-tubulinWT-RNAi-resistant-GFP | Tet-inducible expression of WT gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion |
| 205604 | pCDNA5/FRT/TO–γ-tubulinΔGTP-RNAi-resistant-GFP | Tet-inducible expression of N229A gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion |