nucGEMs probe the biophysical properties of the nucleoplasm
Shu T et al.
bioRxiv 2022
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 203648 | pUBC-Pfv-Sapphire-IRES-H2B-mCherry | Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression. |
| 203649 | pUBC-Pfv-Sapphire | Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter. |
| 203650 | TetCMV-Pfv-Sapphire | Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the doxycycline-inducible promoter. |
| 203651 | pUBC-Pfv-Sapphire_PURO | Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter, with a puromycin selection marker. |
| 203652 | pUBC-SV40NLS-PfV-Sapphire-IRES-H2B-mCherry | Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression. |
| 203653 | pUBC-SV40NLS-Pfv-Sapphire | Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter. |
| 203654 | pUBC-SV40NLS-Pfv-Sapphire-PURO | Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a puromycin selection marker. |
| 203655 | pRS305-LEU2-pINO4-SV40NLS-PfV-GS-Sapphire | Yeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the INO4 promoter. |
| 203656 | pRS305-LEU2-pINO4-SV40NLS-PfV-GS-yEmCherry | Yeast integrative vector for the expression of the yEmCherry-tagged nuclear localizing 40nm nucGEMs under the INO4 promoter. |
| 203657 | pRS305-LEU2-pHIS3-SV40NLS-PfV-GS-GFPMUT3 | Yeast integrative vector for the expression of the GFPMUT3-tagged nuclear localizing 40nm nucGEMs under the HIS3 promoter. |
| 203658 | pRS403-HIS3-pINO4-SV40NLS-PfV-GS-Sapphire | Yeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm GEMs under the HIS3 promoter. |
| 203659 | pINO4-Pfv-Sapphire-HO | Yeast vector encoding donor DNA for integration of 40nm GEMs into the HO locus. |
| 203660 | pINO4-NLS-Pfv-Sapphire-HO | Yeast vector encoding donor DNA for integration of nuclear 40nm GEMs into the HO locus. |