FungalBraid 2.0: expanding the synthetic biology toolbox for the biotechnological exploitation of filamentous fungi.
Moreno-Gimenez E, Gandia M, Saez Z, Manzanares P, Yenush L, Orzaez D, Marcos JF, Garrigues S
Front Bioeng Biotechnol. 2023 Aug 7;11:1222812. doi: 10.3389/fbioe.2023.1222812. eCollection 2023.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 160638 | AtS/MAR10 insulator sequence (GB3458) | pEGB3a1 AtS/MAR10 |
| 203610 | FB271 | Promoter of pyr4 gene from T. reesei. |
| 203611 | FB272 | Coding sequence of pyr4 gene from T. reesei. |
| 203612 | FB273 | Terminator of pyr4 gene from T. reesei. |
| 203613 | FB293 | Assembly of the transcriptional unit for the auxotrophy marker pyr4 from T. reseei. |
| 203614 | FB359 | 5' upstream region of pyrG gene in P. digitatum. |
| 203615 | FB361 | 3' downstream region of pyrG gene in P. digitatum. |
| 203616 | FB372 | Assembly for pyrG deletion in P. digitatum. |
| 203617 | FB413 | Coding sequence for phleomycin resistance. |
| 203618 | FB414 | Coding sequence for terbinafine resistance. |
| 203619 | FB411 | Promoter of Isopenicillin N synthase from P. rubens. |
| 203620 | FB416 | Terminator of acetamidase-encoding gene in A. niger. |
| 203621 | FB430 | TU for the expression of phleomycin resistance. |
| 203622 | FB431 | TU for the expression of terbinafine resistance. |
| 203623 | FB291 | Promoter of the endo-1,4-beta-xylanase A gene from A. nidulans, xylose-inducible. |
| 203624 | FB389 | Promoter of the highly-expressed pyruvate kinase gene from A. niger. |
| 203625 | FB404 | Pomoter of antifungal protein afpB gene from P. digitatum. |
| 203626 | FB405 | Promoter of the glucoamylase gene from A. niger, maltose/starch-inducible. |
| 203627 | FB406 | Promoter of the TAKA-amylase A gene from A. oryzae, maltose/starch-inducible. |
| 203628 | FB407 | Promoter of the elongation factor 1-alpha gene from P. digitatum (PDIG_59570). |
| 203629 | FB408 | Promoter of the ubiquitin ligase gene from P. digitatum (PDIG_07760). |
| 203630 | FB367 | Module for the expression of geneticin resistance and Nanoluciferase. |
| 203631 | FB395 | TU for the expression of luciferase under a synthetic promoter containing the target sequence for gRNA1 (1xLuc). |
| 203632 | FB396 | TU for the expression of luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc). |
| 203633 | FB397 | TU for the expression of luciferase under a synthetic promoter containing three copies of the target sequence for gRNA1 (3xLuc). |
| 203634 | FB398 | Module for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing the target sequence for gRNA1 (1xLuc). |
| 203635 | FB399 | Module for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc). |
| 203636 | FB400 | Module for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing three copies of the target sequence for gRNA1 (3xLuc). |