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FungalBraid 2.0: expanding the synthetic biology toolbox for the biotechnological exploitation of filamentous fungi.
Moreno-Gimenez E, Gandia M, Saez Z, Manzanares P, Yenush L, Orzaez D, Marcos JF, Garrigues S
Front Bioeng Biotechnol. 2023 Aug 7;11:1222812. doi: 10.3389/fbioe.2023.1222812. eCollection 2023. PubMed Article

Plasmids from Article

ID Plasmid Purpose
160638AtS/MAR10 insulator sequence (GB3458)pEGB3a1 AtS/MAR10
203610FB271Promoter of pyr4 gene from T. reesei.
203611FB272Coding sequence of pyr4 gene from T. reesei.
203612FB273Terminator of pyr4 gene from T. reesei.
203613FB293Assembly of the transcriptional unit for the auxotrophy marker pyr4 from T. reseei.
203614FB359 5' upstream region of pyrG gene in P. digitatum.
203615FB3613' downstream region of pyrG gene in P. digitatum.
203616FB372Assembly for pyrG deletion in P. digitatum.
203617FB413Coding sequence for phleomycin resistance.
203618FB414Coding sequence for terbinafine resistance.
203619FB411Promoter of Isopenicillin N synthase from P. rubens.
203620FB416Terminator of acetamidase-encoding gene in A. niger.
203621FB430TU for the expression of phleomycin resistance.
203622FB431TU for the expression of terbinafine resistance.
203623FB291Promoter of the endo-1,4-beta-xylanase A gene from A. nidulans, xylose-inducible.
203624FB389Promoter of the highly-expressed pyruvate kinase gene from A. niger.
203625FB404Pomoter of antifungal protein afpB gene from P. digitatum.
203626FB405Promoter of the glucoamylase gene from A. niger, maltose/starch-inducible.
203627FB406Promoter of the TAKA-amylase A gene from A. oryzae, maltose/starch-inducible.
203628FB407Promoter of the elongation factor 1-alpha gene from P. digitatum (PDIG_59570).
203629FB408Promoter of the ubiquitin ligase gene from P. digitatum (PDIG_07760).
203630FB367Module for the expression of geneticin resistance and Nanoluciferase.
203631FB395TU for the expression of luciferase under a synthetic promoter containing the target sequence for gRNA1 (1xLuc).
203632FB396TU for the expression of luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc).
203633FB397TU for the expression of luciferase under a synthetic promoter containing three copies of the target sequence for gRNA1 (3xLuc).
203634FB398Module for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing the target sequence for gRNA1 (1xLuc).
203635FB399Module for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc).
203636FB400Module for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing three copies of the target sequence for gRNA1 (3xLuc).

Antibodies from Article