WNT signaling memory is required for ACTIVIN to function as a morphogen in human gastruloids.

Yoney A, Etoc F, Ruzo A, Carroll T, Metzger JJ, Martyn I, Li S, Kirst C, Siggia ED, Brivanlou AH
Elife. 2018 Oct 12;7:e38279. doi: 10.7554/eLife.38279. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
203662	BSD-P2A-TagRFP-hSmad1-donor	Donor plasmid for CRISPR knock-in
203663	Puro-T2A-mCitrine-hSmad2-donor	Donor plasmid for CRISPR knock-in