Characterization of RNA editing and gene therapy with a compact CRISPR-Cas13 in the retina.
Kumar S, Hsiao YW, Wong VHY, Aubin D, Wang JH, Lisowski L, Rakoczy EP, Li F, Alarcon-Martinez L, Gonzalez-Cordero A, Bui BV, Liu GS
Proc Natl Acad Sci U S A. 2024 Nov 5;121(45):e2408345121. doi: 10.1073/pnas.2408345121. Epub 2024 Oct 30.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 203442 | pC129: pAAV.CMV-CasRx mCh | Plasmid expressing active RfxCas13d with mCherry reporter for investigating CasRx activity |
| 203443 | pC130: pAAV.CMV-CasRx-VEGFA sgRNA | Plasmid expressing active RfxCas13d with VEGFA mRNA targeting gRNA |
| 203444 | pC131: pAAV.CMV-CasRx-VEGFA sgRNA array | Plasmid expressing active RfxCas13d with an array of three VEGFA mRNA targeting gRNAs |
| 203446 | pM148: pAAV.CMV-Cas13e-NT sgRNA | Plasmid expressing active RfxCas13d with non-targeting gRNA |
| 203447 | pM149: pAAV.CMV-Cas13e-VEGFA sgRNA | Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA |
| 203448 | pM150: pAAV.CMV-Cas13e-VEGFA sgRNA array | Plasmid expressing active Cas13e with an array of three VEGFA mRNA targeting gRNAs |
| 203451 | pM155: pAAV.CMV-Cas13e (GenScript CO)-NT sgRNA | Plasmid expressing active and codon optimised Cas13e with non-targeting gRNA |
| 203452 | pM156: pAAV.CMV-Cas13e (GenScript CO)-VEGFA sgRNA | Plasmid expressing active and codon optimised Cas13e with VEGFA gRNA |
| 204215 | pC178: pAAV.U6.SapI.CMV.Cas13bt3 | Plasmid AAV vector expressing Cas13bt3 and hU6-driven expression of guide RNAs. Contains SapI sites for guide cloning flanked by 5' and 3' full-length DRs. |
| 205744 | pC081: pAAV.CMV.mCherry | AAV vector for expressing CMV-driven mCherry reporter gene. |
| 227800 | pC368: pAAV.CMV-Cas13e-VEGFA sgRNA2 | Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA2 |
| 227801 | pC369: pAAV.CMV-Cas13e-VEGFA sgRNA3 | Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA3 |