Engineering a CRISPRoff Platform to Modulate Expression of Myeloid Cell Leukemia (MCL-1) in Committed Oligodendrocyte Neural Precursor Cells.
Gil M, Hamann CA, Brunger JM, Gama V
Bio Protoc. 2024 Jan 5;14(1):e4913. doi: 10.21769/BioProtoc.4913. eCollection 2024 Jan 5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 203355 | pSB-TRE-CRISPRoff-EF1A-TetOn | Drug-inducible expression of CRISPRoff components needed for DNA methylation and gene repression with Sleeping Beauty backbone |
| 203359 | pSB-BbsI-sgRNA | Contains BbsI cut sites for placement of user-specified sgRNA target sequences with Sleeping Beauty backbone |