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The nematode alpha-catenin ortholog, HMP1, has an extended alpha-helix when bound to actin filaments.

Rangarajan ES, Smith EW, Izard T
J Biol Chem. 2023 Feb;299(2):102817. doi: 10.1016/j.jbc.2022.102817. Epub 2022 Dec 17. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
186436pGEX-6P-HMP1_FABDExpresses the HMP1 F-actin Binding domain (677-927) with an N-terminal GST tag and a PRescission Protease cleavage site
191740pGEX-6P--Dr-aCateninFLExpresses FL D rerio catenin in bacteria
203333pET28-His8-aCat(660-906)Bacterial expression of alpha-catenin F-actin binding domain
203334pET28-His8-aCat(660-885)Bacterial expression of alpha-catenin F-actin binding domain
203335pET28-His8-aCat(660-865)Bacterial expression of alpha-catenin F-actin binding domain

Antibodies from Article