Quantitative analysis of autophagy reveals the role of ATG9 and ATG2 in autophagosome formation.
Broadbent DG, Barnaba C, Perez GI, Schmidt JC
J Cell Biol. 2023 Jul 3;222(7):e202210078. doi: 10.1083/jcb.202210078. Epub 2023 Apr 28.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 203222 | Halo-ATG9A HRD | Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG9A locus in human cells |
| 207539 | Halo-LC3B HRD | Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous LC3B locus in human cells |
| 207542 | Halo-ATG5 HRD | Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG5 locus in human cells |
| 207545 | SNAP-LC3 HRD | Homologous recombination donor to integrate a SNAP-Tag at the endogenous LC3 locus in human cells |
| 207548 | ULK1-Halo HRD | Homologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cells |
| 207549 | AAVS1 LAMP1-mNeonGreen HRD | Homologous recombination donor to integrate and expression cassette for LAMP1-mNEONGREEN in the AAVS1 locus of human cells |
| 207551 | AAVS1 EGFP-LC3B HRD | Homologous recombination donor to integrate and expression cassette for eGFP-LC3B in the AAVS1 locus of human cells |
| 207552 | AAVS1 EGFP-GABARAPL1 HRD | Homologous recombination donor to integrate and expression cassette for EGFP-GABRAPL1 in the AAVS1 locus of human cells |
| 207553 | AAVS1 EGFP-Sec61B | Homologous recombination donor to integrate and expression cassette for EGFP-Sec61B in the AAVS1 locus of human cells |
| 207554 | WIPI2 sgRNA | pX330 expressing Cas9 and a sgRNA targeting the WIPI2 locus |
| 207555 | ATG5 sgRNA | pX330 expressing Cas9 and a sgRNA targeting the ATG5 locus |
| 207557 | ATG9A sgRNA | pX330 expressing Cas9 and a sgRNA targeting the ATG9A locus |
| 207558 | ATG13 sgRNA | pX330 expressing Cas9 and a sgRNA targeting the ATG13 locus |
| 207559 | ULK1 sgRNA | pX330 expressing Cas9 and a sgRNA targeting the ULK1 locus |
| 207561 | ULK1 KO sgRNA | pX330 expressing Cas9 and a sgRNA targeting the ULK1 locus |
| 207563 | ATG16L1 sgRNA | pX330 expressing Cas9 and a sgRNA targeting the ATG16L1 locus |
| 207580 | AAVS1 SNAP-Sec61B | Homologous recombination donor to integrate and expression cassette for SNAPtag-Sec61B in the AAVS1 locus of human cells |