Three-step docking by WIPI2, ATG16L1, and ATG3 delivers LC3 to the phagophore.
Rao S, Skulsuppaisarn M, Strong LM, Ren X, Lazarou M, Hurley JH, Hummer G
Sci Adv. 2024 Feb 9;10(6):eadj8027. doi: 10.1126/sciadv.adj8027. Epub 2024 Feb 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 197460 | 2BT-His-TEV-cs-LC3b | Bacterial Expression of human LC3b |
| 202777 | His-ATG3 | ATG3 protein (WT) overexpression in E.Coli. 6His-TEVsite-ATG3 WT-stop |
| 202778 | His-ATG3-C264A | ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-C264A-stop |
| 202779 | His-ATG3-Y209A | ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-Y209A-stop |
| 202780 | His-ATG3-Y210A | ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-Y210A-stop |
| 202781 | His-ATG3-T244A | ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-T244A-stop |
| 202782 | His-ATG3-H262A | ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-H262A-stop |
| 202783 | His-ATG3-R265A | ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-R265A-stop |
| 202784 | His-ATG3-H266A | ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-H266A-stop |
| 202785 | His-ATG3-K208D | ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-K208D-stop |
| 202786 | His-ATG3-K62D/K64D | ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-K62D/K64D-stop |
| 212027 | pMX-IG-ATG3 (H262A) | Expresses untagged ATG3 (H262A) in mammalian cells |
| 212031 | pMX-IG-ATG3 (K62D/K64D) | Expresses untagged ATG3 (K62D/K64D) in mammalian cells |