Hominoid SVA-lncRNA AK057321 targets human-specific SVA retrotransposons in SCN8A and CDK5RAP2 to initiate neuronal maturation.

Nadler MJS, Chang W, Ozkaynak E, Huo Y, Nong Y, Boillot M, Johnson M, Moreno A, Matthew P Anderson
Commun Biol. 2023 Mar 30;6(1):347. doi: 10.1038/s42003-023-04683-8. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
202800	pLV-EFalpha-SVA-lncRNA-AK057321-mcherry-puro	Lentiviral vector to express SVA-lncRNA AK057321 with ires for mcherry and puro expression
202813	pLVX-SVA-lncRNA-AK057321-mcherry	Lentiviral vector to express of SVA-lncRNA AK057321 with CMV for mcherry expression
202818	pLVX-5'-aptamer-tagged-SVA-lncRNA-AK057321-mcherry	Lentiviral vector to express 5'-aptamer tagged SVA-lncRNA AK057321 with CMV for mcherry expression
202821	lentiCRISPRv2-sgRNAs-del-AK057321-SVA-mcherry	Lentiviral vector modified to express two sgRNAs that delete the SVA within the SVA-lncRNA AK057321 gene (exon 3) with EF-1apha for Cas9 and mcherry expression
202822	lentiCRISPRv2-sgRNAs-del-ZNF91-mcherry	Lentiviral vector modified to express two sgRNAs that delete exon 1 within the ZNF91 gene with EF-1apha for Cas9 and mcherry expression
202823	lentiCRISPRv2-sgRNAs-del-ZNF91-BFP	Lentiviral vector modified to express two sgRNAs that delete exon 1 within the ZNF91 gene with EF-1apha for Cas9 and BFP expression
202824	lentiCRISPRv2-sgRNAs-del-CDK5RAP2-SVA-BFP	Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CDK5RAP2 gene with EF-1apha for Cas9 and BFP expression
202825	lentiCRISPRv2-sgRNAs-del-CHAF1B-SVA-BFP	Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CHAF1B gene with EF-1apha for Cas9 and BFP expression
202826	lentiCRISPRv2-sgRNAs-del-SCN8A-SVA-BFP	Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the SCN8A gene with EF-1apha for Cas9 and BFP expression
202827	pGL3-luciferase-reporter-AGPAT3-SVA	pGL3 luciferase reporter with AGPAT3 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression
202828	pGL3-luciferase-reporter-CDK5RAP2-SVA	pGL3 luciferase reporter with CDK5RAP2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression
202829	pGL3-luciferase-reporter-CHAF1B-SVA	pGL3 luciferase reporter with CHAF1B SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression
202830	pGL3-luciferase-reporter-CHAF1B-SVA-del-VNTR	pGL3 luciferase reporter with CHAF1B-SVA-del-VNTR sequence cloned upstream of SV40 promoter to test effects of SVA-del-VNTR insertion on luciferase expression
202831	pGL3-luciferase-reporter-POFUT2-SVA	pGL3 luciferase reporter with POFUT2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression
202832	pGL3-luciferase-reporter-JAM2-SVA	pGL3 luciferase reporter with JAM2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression
202833	pGL3-luciferase-reporter-HTT-SVA	pGL3 luciferase reporter with HTT SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression
202834	pLVX-SVA-lncRNA-AK057321 shRNA 1A	Lentiviral vector that expresses an shRNA that knocks down SVA-lncRNA AK057321 expression
202835	SVA-lncRNA AK057321 shRNA 1A scramble control	Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 1A
202836	pLVX-SVA-lncRNA AK057321 shRNA 1S	Lentiviral vector that expresses an shRNA that knocks down SVA-lncRNA AK057321 expression
202837	SVA-lncRNA AK057321 shRNA 1S scramble	Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 1S
202838	pLVX-SVA-lncRNA AK057321 shRNA 2S	Lentiviral vector that expresses an shRNA that knocks down SVA-lncRNA AK057321 expression
202839	SVA-lncRNA AK057321 shRNA 2S scramble	Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 2S
202840	TetO-CDK5RAP2	Doxycycline inducible expression of CDK5RAP2 cDNA