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All-optical mapping of cAMP transport reveals rules of sub-cellular localization

Xiang KM, Park P, Koren SA, Hayward RF, Cohen AE
bioRxiv 2023 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
202716pLenti-CMV-Pink Flamindo-p2a-bPACMammalian co-expression of soluble Pink Flamindo, a fluorescent cAMP indicator and soluble bPAC, a photoactivatable adenylyl cyclase
202717pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPACMammalian co-expression of membrane-bound Pink Flamindo and soluble bPAC for cAMP perturbation and imaging
202718pLenti-CMV-Pink Flamindo-p2a-paGFP-bPAC-CAAXMammalian co-expression of soluble Pink Flamindo and membrane-bound bPAC for cAMP perturbation and imaging
202719pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC-CAAXMammalian co-expression of membrane-bound Pink Flamindo and membrane-bound bPAC for cAMP perturbation and imaging
204669pLenti-CMV-Myr-Pink FlamindoMammalian expression of membrane-bound Pink Flamindo for cAMP imaging
204670pLenti-CMV-paGFP-bPACMammalian expression of bPAC tagged with paGFP for cAMP production
204671pLenti-CMV-paGFP-bPAC-CAAXMammalian expression of membrane-bound bPAC tagged with paGFP for cAMP production

Antibodies from Article