Light-inducible protein degradation in E. coli with the LOVdeg tag.
Tague N, Coriano-Ortiz C, Sheets MB, Dunlop MJ
Elife. 2024 Jan 25;12:RP87303. doi: 10.7554/eLife.87303.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
202712 | pBbS5c-mCherry-AsLOV2*(543) | Expresses an IPTG inducible mCherry fused to a blue light responsive degradation tag (LOVdeg) |
210863 | pBbA5k-AcrAB-AsLOV2*(543) | IPTG inducible promoter (lacUV5) expressing AcrA and AcrB-AsLOV2*(543). AsLOV2*(543) is also described in the literature as LOVdeg. |