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Light-inducible protein degradation in E. coli with the LOVdeg tag.

Tague N, Coriano-Ortiz C, Sheets MB, Dunlop MJ
Elife. 2024 Jan 25;12:RP87303. doi: 10.7554/eLife.87303. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
202712pBbS5c-mCherry-AsLOV2*(543)Expresses an IPTG inducible mCherry fused to a blue light responsive degradation tag (LOVdeg)
210863pBbA5k-AcrAB-AsLOV2*(543)IPTG inducible promoter (lacUV5) expressing AcrA and AcrB-AsLOV2*(543). AsLOV2*(543) is also described in the literature as LOVdeg.

Antibodies from Article