Widefield imaging of rapid pan-cortical voltage dynamics with an indicator evolved for one-photon microscopy.
Lu X, Wang Y, Liu Z, Gou Y, Jaeger D, St-Pierre F
Nat Commun. 2023 Oct 12;14(1):6423. doi: 10.1038/s41467-023-41975-3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 202606 | pcDNA3.1/Puro-CAG-JEDI-1P-P2A-mCherry-CAAX | Genetically encoded voltage indicator (GEVI) JEDI-1P fused to membrane-anchored mCherry via P2A, expressed under strong mammalian promoter (CAG) |
| 202607 | pcDNA3.1/Puro-CAG-JEDI-1P | Genetically encoded voltage indicator (GEVI) JEDI-1P, expressed under strong mammalian promoter (CAG) |
| 202608 | pAAV-EF1a-DIO-JEDI-1P-Kv-WPRE | Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector expressed under the mammalian promoter (EF1a) |
| 202610 | pAAV-EF1a-DIO-tdTomato-WPRE | Double floxed red fluorescent protein tdTomato in AAV production vector expressed under the mammalian promoter (EF1a) |
| 202611 | pAAV-EF1a-DIO-JEDI-1P-WPRE | Double floxed genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector expressed under the mammalian promoter (EF1a) |
| 202612 | pAAV-hSyn-JEDI-1P-Kv-WPRE | Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for neuron-specific expression using the promoter hSyn |
| 202613 | pAAV-hSyn-JEDI-1P-WPRE | Genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for neuron-specific expression using the promoter hSyn |
| 202614 | pAAV-CaMKII-JEDI-1P-Kv-WPRE | Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKII |
| 202615 | pAAV-CaMKII-JEDI-1P-WPRE | Genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKII |
| 202616 | pAAV-GFAP-JEDI-1P-WPRE | Genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for astrocytes specific expression using the promoter GFAP |
| 202617 | pAAV-EF1a-FRT-JEDI-1P-Kv-WPRE | Genetically encoded voltage indicator (GEVI) JEDI-1P flanked by FRT in AAV production vector expressed under the mammalian promoter (EF1a), FLP recombinase-dependent |
| 202618 | pJFRC7-JEDI-1P | Genetically encoded voltage indicator (GEVI) JEDI-1P under the promoter hsp70 for Drosophila (insect) expression |
| 202619 | pHUC-JEDI-1P | Genetically encoded voltage indicator (GEVI) JEDI-1P under the HuC promoter for pan neuronal expression in zebrafish; JEDI-1P sequence codon-optimized for zebrafish expression |
| 202620 | pAAV-EF1a-DIO-mCherry-WPRE | Double floxed red fluorescent protein mCherry in AAV production vector expressed under the mammalian promoter (EF1a) |