Skip to main content
Addgene

Condensation of LINE-1 is critical for retrotransposition.

Sil S, Keegan S, Ettefa F, Denes L, Boeke JD, Holt LJ
Elife. 2023 Apr 28;12:e82991. doi: 10.7554/eLife.82991. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
202566pCEP-Puro-TetOn-L1RP-ORF1_mNeonGreen2Expresses an endogenous-like L1RP LINE-1 element with a C-terminal mNeonGreen2 fluorophore on the ORF1 protein for fluorescence microscopy
202568pCEP-Puro-TetOn-L1RP-ORF1_K3AK4A_Halo-GFPaiExpresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the K3A and K4A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR
202569pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPaiExpresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR
202571pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPaiExpresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR
202585pETM11-6xHis-TEV-hORF1p_K3AK4AAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar
202586pETM11-6xHis-TEV-hORF1p_R261AAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar
202587pETM11-6xHis-TEV-hORF1p_StammerAAAAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification
202588pETM11-6xHis-TEV-hORF1p_StammerAEAAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification

Antibodies from Article