BLINCAR: a reusable bioluminescent and Cas9-based genetic toolset for repeatedly modifying wild-type Scheffersomyces stipitis.
Reichard WD, Smith SE, Robertson JB
mSphere. 2023 Aug 24;8(4):e0022423. doi: 10.1128/msphere.00224-23. Epub 2023 Jun 22.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 202560 | pAllet | E. coli cloning vector with large MCS (50+ unique restriction sites) |
| 202645 | pWR9 | episomal bioluminescent reporter plasmid for S. stipitis |
| 202710 | pWR10 | episomal bioluminescent reporter plasmid for S. stipitis |
| 202711 | pWR16 | integrating bioluminescent reporter plasmid for S. stipitis |
| 202770 | pWR23 | episomal bioluminescent reporter plasmid for S. stipitis |
| 202771 | pWR24 | episomal bioluminescent reporter plasmid for S. stipitis |
| 202776 | pWR76 | episomal fluorescent reporter plasmid for S. stipitis |
| 202795 | pWR58 | integrating bioluminescene reporter for Cas9 activity in S. stipitis |
| 203330 | pWR54 | episomal bioluminescent reporter plasmid for S. stipitis |
| 203331 | pWR63 | episomal Cas9 and sgRNA expression vector without sgRNA for S. stipitis |
| 203336 | pWR63(YUM1) | episomal Cas9 and YUM1-targeting sgRNA expression vector for S. stipitis |
| 203422 | pWR88 | empty BLINCAR plasmid for payload delivery, no payload, no genomic target |
| 203423 | pWR89 | empty BLINCAR plasmid for payload delivery, no payload, integrates at EGC3 locus |
| 203914 | pWR90 | empty BLINCAR plasmid for payload delivery, no payload, integrates at GSC2 locus |
| 203915 | pWR91 | empty BLINCAR plasmid for payload delivery, no payload, integrates at CRF1 locus |
| 203916 | pWR95 | BLINCAR plasmid for gene deletion, no genomic target |
| 203917 | pWR95(URA3) | BLINCAR plasmid for gene deletion, URA3 target |