TAC-TIC, a high-throughput genetics method to identify triggers or blockers of bacterial toxin-antitoxin systems.

Bobonis J, Yang ALJ, Voogdt CGP, Typas A
Nat Protoc. 2024 May 9. doi: 10.1038/s41596-024-00988-y. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
202602	pJB37	Arabinose inducible expression vector expressing retron Rcat-Sen2
202601	pJB158	RP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.