Scalable, robust, high-throughput expression & purification of nanobodies enabled by 2-stage dynamic control.
Hennigan JN, Menacho-Melgar R, Sarkar P, Golovsky M, Lynch MD
Metab Eng. 2024 Sep;85:116-130. doi: 10.1016/j.ymben.2024.07.012. Epub 2024 Jul 24.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 202462 | pSMART-HCKan-yibDp-roGFP2 | Expresses redox sensitive GFP (roGFP) after phosphate depletion |
| 202463 | pETM6-Amp-T7-GFPuv | Expresses GFPuv with T7 induction |
| 202464 | pETM6-Amp-T7-roGFP | Expresses redox sensitive GFP (roGFP) with T7 induction |
| 202465 | pCASCADE-LCAmp-trxB | Expresses a guide RNA to silence trxB after phosphate depletion |
| 202466 | pSMART-HCKan-EV | Empty vector of pSMART-HCKan |
| 202467 | pSMART-HCKan-yibDp-VHH72 | Expresses the nanobody VHH72 after phosphate depletion |
| 202468 | pSMART-HCKan-yibDp-VHH-GFPenhancer | Expresses the nanobody GFP enhancer after phosphate depletion |
| 202469 | pSMART-HCKan-yibDp-VHH-GFPminimizer | Expresses the nanobody GFP minimizer after phosphate depletion |
| 202470 | pSMART-HCKan-yibDp-VHH-H6antivenin | Expresses the nanobody H6 antivenin after phosphate depletion |
| 202471 | pSMART-HCKan-yibDp-VHH-VCAM1 | Expresses the nanobody VCAM1 after phosphate depletion |
| 202472 | pSMART-HCKan-yibDp-VHH-1B5 | Expresses the nanobody 1B5 after phosphate depletion |
| 202473 | pSMART-HCKan-yibDp-VHH-2E7 | Expresses the nanobody 2E7 after phosphate depletion |
| 202474 | pSMART-HCKan-yibDp-VHH-3E3 | Expresses the nanobody 3E3 after phosphate depletion |
| 202475 | pSMART-HCKan-yibDp-VHH-C9BoNT-A | Expresses the nanobody C9 BoNT-A after phosphate depletion |
| 202476 | pSMART-HCKan-yibDp-VHH-E7BoNT-A | Expresses the nanobody E7 BoNT-A after phosphate depletion |
| 202477 | pSMART-HCKan-yibDp-VHH-JM3 | Expresses the nanobody JM3 after phosphate depletion |
| 202478 | pSMART-HCKan-yibDp-VHH-J3 | Expresses the nanobody J3 after phosphate depletion |
| 202479 | pSMART-HCKan-yibDp-VHH-m36.4 | Expresses the nanobody m36.4 after phosphate depletion |
| 202480 | pSMART-HCKan-yibDp-VHH-Re5D06 | Expresses the nanobody Re5D06 after phosphate depletion |
| 202481 | pSMART-HCKan-yibDp-VHH-Re9F06 | Expresses the nanobody Re9F06 after phosphate depletion |
| 202482 | pCOLA-Gent-ppiB | Expresses peptidyl-prolyl cis-trans isomerase (ppiB) after phosphae depletion |
| 202483 | pCOLA-Gent-yibDp-Erv1p | Expresses sulfydryl oxidase (Erv1p) after phosphate depletion |
| 202484 | pCOLA-Gent-EM7-Erv1p | Expresses sulfhydryl oxidase (Erv1p) constitutively |
| 202485 | pCOLA-Gent-EM7-Erv1p-DsbC | Expresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutively |
| 202486 | pCOLA-Gent-EM7-Erv1p-PDI | Expresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutively |
| 202487 | pETM6-T7-VHH72 | Expresses the nanobody VHH72 with T7 induction |
| 202488 | pETM6-T7-VHH-GFPminimizer | Expresses the nanobody GFP minimizer with T7 induction |
| 202490 | pETM6-T7-VHH-C9BoNT-A | Expresses the nanobody C9 BoNT/A with T7 induction |
| 202491 | pCOLA-Gent-EV | pCOLA-Gent Empty Vector |