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Hardwiring tissue-specific AAV transduction in mice through engineered receptor expression.

Zengel J, Wang YX, Seo JW, Ning K, Hamilton JN, Wu B, Raie M, Holbrook C, Su S, Clements DR, Pillay S, Puschnik AS, Winslow MM, Idoyaga J, Nagamine CM, Sun Y, Mahajan VB, Ferrara KW, Blau HM, Carette JE
Nat Methods. 2023 Jul;20(7):1070-1081. doi: 10.1038/s41592-023-01896-x. Epub 2023 Jun 8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
202459pattB-LSL-AAVR-F2A-spCas9The plasmid backbone used to generate the recombination template to generate the SELECTIV mice through Integrase Mediated Transgenesis. Allows for Cre-dependent expression of AAVR and Cas9.

Antibodies from Article